| Technologies > Bio Technology |
| Bio Technology(Biosensor & Biochip) |
| What is SPR? |
The Surface Plasmon Resonance (SPR) is an optical technology for monitoring specific binding events between two or more bio-molecules without the use of labels. For recent 10 years, its reliability has been proven within life science applications.
The SPR signal originates in changes in the refractive index at the surface layer, caused by concentration changed on the sensor chip surface with adsorbed bio-molecules.
The SPR is detected by measurement of the intensity of reflected light. From the SPR angle, a sharp decrease or dip in intensity can be measured.
Any change in thickness or index of refraction of the material adsorbed onto gold surface results in a shift of the SPR angle. The SPR angle shift is used as a response unit to quantify binding molecules to the sensor surface. |
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| SPR Sensor Principles |
Surface refractive index is monitored over time as binding events occur. |
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| Scheme of SPR Imaging System |
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Schematic of SPR imaging. A parallel beam is incident on a prism near the SPR resonance angle. The reflected light is detected by a CCD Array. Contrast in SPR image arises from variation in the reflected light intensity DR. |
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| Major Application |
| In-Situ SAM Formation analysis |
● Real Time monitoring of SAM formation
● Optical Chracterization of SAM (determination of n,k,d of layer)
● Protein and DNA Linker Chracterization |
| Functional Analysis of Biomolecular Interaction |
● DNA immobilization & Hybridization Monitoring
● Real Time Monitoring of Biomolecule Interaction on Recognition Interface.
● Proteomics Identification, Ligand Fishing , Identity Binding Partners |
| Organic Thin Film Chracterization |
● Surface Chemistry
● Nano-level Research in Supramolecular Chemistry
● Polymer Chemistry |
| Food or Bio Processing Monitoring |
● Quality control, Process control
● Monitoring of Microorganism in Water |
